Quantitative ELISA kits for hematology, metabolism, neuroscience, oncology and endocrinology research. Tell us the target and species and we confirm the kit, its detection range and the sample types it has been validated for.
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The range covers quantitative ELISA kits for human, mouse and rat samples, and kits for other species, such as sheep, cattle, pigs and chickens, for veterinary and comparative research. Tell us the species and we check which targets are available. Most kits are supplied as a pre-coated 96-well plate with everything needed for one standard curve and up to about 40 samples in duplicate.
| Research area | Typical targets | Common samples |
|---|---|---|
| Hematology | Erythropoietin (EPO), ferritin, coagulation factors | Serum, plasma |
| Metabolism | Insulin, adiponectin, leptin, cyclic GMP (cGMP) | Serum, plasma, tissue homogenate |
| Neuroscience | α-synuclein, BDNF, tau, neuropeptides | CSF, serum, brain homogenate |
| Oncology | VEGF, TIMP-1, NF-κB pathway proteins | Serum, cell culture supernatant, cell lysate |
| Endocrinology | ACTH, cortisol, thyroid hormones, testosterone | Serum, plasma, saliva |
Most mismatches between a kit and an experiment come from one of these eight checks. Work through them in order before you order.
In a sandwich ELISA the target is captured by one antibody on the plate and detected by a second antibody against a different site. Signal rises with concentration. It is the most specific format, and the usual choice for proteins such as cytokines, growth factors and hormones above a few kilodaltons.
In a competitive ELISA the target in your sample competes with a labelled or coated version of itself for a limited amount of antibody. Signal falls as concentration rises. Small molecules with only one binding site, such as cGMP, cortisol and short peptides, are measured this way.
| Sandwich | Competitive | |
|---|---|---|
| Best for | Proteins with two epitopes | Small molecules and peptides |
| Signal vs concentration | Increases | Decreases |
| Antibodies needed | Matched capture and detection pair | One antibody |
| Typical targets | TIMP-1, VEGF, EPO, insulin | cGMP, cortisol, testosterone |
Sample handling causes more ELISA failures than the kit itself. The table summarises the essentials; the full method for each sample is in the ELISA sample preparation guide.
| Sample | Preparation | Watch for |
|---|---|---|
| Serum | Clot at room temperature, centrifuge, aliquot | Haemolysis, repeated freeze-thaw |
| Plasma | EDTA, heparin or citrate tube; centrifuge within 30 minutes | Anticoagulant listed as compatible on the datasheet |
| Cell culture supernatant | Centrifuge to remove cells and debris | Serum proteins in the medium |
| Cell or tissue lysate | Lyse on ice with protease inhibitors, clarify, measure protein | Detergents the kit does not tolerate |
| Saliva, urine, CSF | Centrifuge; dilute as the manual directs | Low concentrations and matrix effects |
For lysates, the guide to lysis buffers explains which detergents are gentle enough for immunoassays.
Contents vary by manufacturer, and the kit manual we send lists them. A typical sandwich kit includes:
You supply a plate reader at 450 nm, calibrated pipettes, a plate washer or wash bottle, deionised water and, for most kits, an incubator at 37 °C.
Send the target or catalogue number, species, sample type and the number of plates. We reply with the kit manual or datasheet, the price for your quantity, lead time and how the kit ships to your country. Kits that need cold-chain shipping travel at 2–8 °C.
Planning a full study? Pair the kits with antibodies for western blot or IHC to confirm what the ELISA shows, or with enzyme activity and cell assays for function.
Only if the datasheet shows cross-reactivity data for that species. Kits use antibodies raised against one species' protein, and sequence differences often stop them binding another species' version. Use a kit validated for your species, or send us the catalogue number and we check the cross-reactivity data.
Sensitivity, or the lowest detectable dose, is the smallest concentration the kit can tell apart from zero. The detection range is the span of the standard curve where results are quantitative. Your samples, after any dilution, should sit inside the detection range, ideally near its middle.
A standard curve of seven points plus a blank, run in duplicate, takes 16 wells. That leaves 80 wells, or 40 samples in duplicate. Duplicates are the usual minimum for quantitative results.
Most kits ship and are stored at 2–8 °C, and some components need -20 °C. Store each component as the manual says on arrival and use the kit before its expiry date. We confirm cold-chain shipping for your country when we quote.
Kits in this range are generally supplied for research use only. If you need a kit for clinical diagnosis, tell us the country and intended use and we will confirm what is available and how it is registered there.
ELISA kits
Every ELISA format detects an antigen with an antibody and an enzyme, but they suit different targets. Here is how each works, which one a quantitative kit is likely to use, and how to read the results.
5 minute read
ELISA kits
Most failed ELISAs fail before the plate is opened. This guide covers collection, centrifugation, storage and dilution for each common sample type, and the checks that prove your matrix is not skewing the result.
4 minute read
ELISA kits
High background usually means washing or detection reagent; no signal usually means a step, a reagent or the sample. Here is how to tell which, using the wells you already ran.
4 minute read
Send the details on the right with your list. Orders often combine ranges, so add anything else you need and we quote it together.
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What to tell us
Product names or catalogue numbers, quantities and the delivery country. We'll confirm availability, lead time and the documents that ship with the order.